From the AGA Journals

Identification of subtypes, mutations in CRC and colon cancer greatly increases survival rates

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Tumor genotyping should be important in colon cancer treatment

It is now recognized that colon cancer is quite heterogeneous on a genetic level, and the clinical features associated with each of these genetic subtypes are equally heterogeneous. The two current studies addressed the question of whether long-term prognosis differs among these genetic subtypes. Colon tumors were first divided into five distinct categories, based upon a panel of multiple molecular markers. One study analyzed more than 2,700 colon cancers of all stages, whereas the other examined more than 2,700 stage III tumors from a North Central Cancer Treatment Group adjuvant chemotherapy trial.

Similar patterns emerged. Tumors with the least favorable prognosis were the so-called “serrated” tumors that are DNA mismatch-repair (MMR) proficient and are positive for a BRAF mutation. Tumors with deficient MMR (MSI-H), whether sporadic or associated with Lynch syndrome, consistently exhibited the most favorable prognosis and highest rates of long-term survival.

These studies provide strong evidence that links survival with specific tumor genotypes, regardless of stage or treatment, and establish the significance of molecular genotyping for prognostic purposes. There are other important reasons to perform tumor genotyping, including the identification of unrecognized Lynch syndrome. However, the therapeutic implications of tumor genotyping remain less clear, as meaningful targeted therapies for each of the specific subgroups are still lacking. In particular, effective targeting of the BRAF oncogene in serrated tumors remains an important priority. More refined molecular classifications are likely to emerge in the future, and the opportunities to offer more precise and personalized approaches to management should increase in parallel.

Dr. Daniel C. Chung is associate professor, department of medicine, Harvard Medical School, Boston, and director, Hi-Risk Gastrointestinal Cancer Clinic, Massachusetts General Hospital, also in Boston. He had no conflicts of interest.


 

FROM GASTROENTEROLOGY

References

Identifying subtypes and tumor mutations in patients with colorectal and stage III colon cancer can significantly improve survival rates, according to two new studies published in the January issue of Gastroenterology (doi:10.1053/j.gastro.2014.09.038 and doi:10.1053/j.gastro.2014.09.041).

In the first study, researchers found that etiologically defined subtypes of colorectal cancer are characterized by marked differences in survival rates and confirmed the clinical importance of studying the molecular heterogeneity of the disease.

“Increasing evidence indicates that colorectal cancer (CRC) is a biologically heterogeneous disease that can develop via a number of distinct pathways involving different combinations of genetic and epigenetic changes,” wrote Amanda Phipps, Ph.D, of the University of Washington in Seattle, and her coinvestigators, adding that “the biologic distinctions between CRC subtypes resulting from different etiologic pathways may plausibly translate to differences in survival.”

Between 1998 and 2007, 2,706 participants were enrolled for this study through the population-based Seattle Colon Cancer Family Registry and followed for survival through 2012. Of those, 2,050 had tumor samples collected from them, each of which was classified into one of five subtypes based on tumor markers: type 1 (microsatellite instability [MSI] high, CpG island methylator phenotype [CIMP] positive, positive for BRAF mutation, negative for KRAS mutation); type 2 (microsatellite stable [MSS] or MSI-low, CIMPpositive, positive for BRAF mutation, negative for KRAS mutation); type 3 (MSS or MSI-low, non-CIMP, negative for BRAF mutation, positive for KRAS mutation); type 4 (MSS or MSI-low, non-CIMP, negative for mutations in BRAF and KRAS); and type 5 (MSI-high, non-CIMP, negative for mutations in BRAF and KRAS).

To analyze data, Cox regression models were used to estimate hazard ratios, 95% confidence intervals, and associations for each subtype with specific diseases and overall mortality, all of which were adjusted for age, sex, body mass, diagnosis year, and smoking history.

Results indicated that type 4 tumors were the most predominant, but subjects with type 2 tumors had the highest disease-specific mortality (hazard ratio = 2.20) and subjects with type 3 tumors also had unusually high disease-specific mortality (HR = 1.32). Type 5 tumors were associated with the lowest disease-specific mortality (HR = 0.30). Associations with overall mortality were similar to those with disease-specific mortality.

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