Original Research

In Vitro and In Situ Characterization of Arthroscopic Loop Security and Knot Security of Braided Polyblend Sutures: A Biomechanical Study

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We conducted a study to evaluate biomechanical performance during destructive testing of several different suture materials in various arthroscopic knot configurations under both in vitro and in situ conditions. Surgeons of different levels of experience tied the knots.

Three different arthroscopic knots (static surgeon’s, Weston, Tennessee slider) with 3 reverse half-hitches on alternating posts were tested using Fiberwire, ForceFiber, Orthocord, and Ultrabraid suture materials under both in vitro and in situ (blood plasma at 37°C) conditions. Three surgeons of different experience levels tied the knots on a post 30 mm in circumference. A single load-to-failure test was performed.

There were no significant in vitro–in situ differences for Ultrabraid in the different knot configurations or with the different experience levels. Surgeon B (intermediate experience) showed no significant differences between test conditions for any knot configuration or suture material. With Tennessee slider knots, surgeon C (least experience) showed significantly lower clinical failure load under both test conditions and had a higher percentage of complete knot slippage. Surgeon B had no knot slippage with use of Fiberwire.

Both the aqueous environment and the surgeon’s familiarity with certain knots have an effect on knot security.


 

References

Open-surgery knot tying is easily learned and performed, but knot tying during arthroscopic procedures can be both challenging and frustrating. According to Burkhart and colleagues,1,2 knot security is defined as the effectiveness of the knot in resisting slippage when load is applied, whereas loop security is the effectiveness in maintaining a tight suture loop while a knot is being tied. Arthroscopic knots commonly begin with an initial slipknot locked in place with a series of half-hitches. During arthroscopic surgery, the surgeon usually must tie an arthroscopic knot to obtain secure tissue fixation, an essential component of soft-tissue repair. A secure knot provides optimal tissue apposition for healing, which will ultimately improve functional outcome. For a knot to be effective, it must have both knot security and loop security. Knot security depends on knot configuration, the coefficient of friction, ductility, handling properties, solubility and diameter of suture material, internal interference, slack between throws, and surgeon experience. Tissue fluid and tissue reaction to suture material may affect knot and loop security.

The ideal knot would be easy to tie and reproducible and would not slip or stretch before tissue is healed. The ideal suture material should provide adequate strength to hold soft tissue in an anatomically correct position until healing can occur. It should also be easily and efficiently manipulated by arthroscopic means when tissues are being secured with knots and secure suture loops. Studies have been conducted to evaluate the security of knots tied with arthroscopic techniques, knot configurations, and suture materials, and these investigations have often evaluated knot performance under single load-to-failure (LTF) test scenarios and cyclic loading in vitro (dry environment) in a room-temperature environment.2-10 To our knowledge, few if any attempts have been made to simulate in situ conditions at body temperature when testing knot security. The fluid environment and the temperature could potentially affect the effectiveness of knots, as knot security depends on friction, internal interference, and slack between throws.1

We conducted a study to evaluate biomechanical performance (knot security, loop security) during destructive testing of several different suture materials with various arthroscopic knot configurations. The study was performed under in vitro (dry environment) and in situ (wet environment) conditions by surgeons with different levels of experience.

Materials and Methods

This investigation was conducted at the Orthopaedic Research Institute at Via Christi Health in Wichita, Kansas. The study compared 4 different suture materials tied with 3 different commonly used arthroscopic knots by 3 surgeons with different levels of experience. The 4 types of braided polyblend polyethylene sutures were Fiberwire (Arthrex, Naples, Florida), ForceFiber (Stryker, San Jose, California), Orthocord (DePuy-Mitek, Warsaw, Indiana), and Ultrabraid (Smith & Nephew, Memphis, Tennessee). Each suture material was tied with 3 arthroscopic knots—static surgeon’s knot, Weston knot,11 Tennessee slider12—and a series of 3 reversing half-hitches on alternating posts (RHAPs) (Figure 1). These knots were chosen based on studies showing they have a higher maximum force to failure when combined with 3 RHAPs.1,2,5,9,13-17

We evaluated performer variability with the help of 3 investigator-surgeons who differed in their level of experience tying arthroscopic knots. This experience was defined on the basis of total number of arthroscopies performed—one of the most important factors predicting basic arthroscopic skills. Our surgeon A was a sports medicine fellowship–trained surgeon with 10 years of experience and a significant number of arthroscopies performed annually (350); surgeon B was a sports medicine fellowship–trained surgeon with 3 years of experience and an annual arthroscopy volume of more than 250 procedures; and surgeon C was a third-year orthopedic resident with about 100 arthroscopies performed.

All knots were tied on a standardized post 30 mm in circumference, which provided a consistent starting circumference for each knot and replicated the suture loop created during arthroscopic rotator cuff repair. All knots were tied using standard arthroscopic techniques, with a standard knot pusher and a modified arthroscopic cannula, in a dry environment (Figure 2). Servohydraulic materials testing system instruments (model 810; MTS Systems, Eden Prairie, Minnesota) were used to test the knot security and loop security of each combination of knots and suture types. Two round hooks (diameter, 3.9 mm) were attached to the actuator and the load cell (Figure 3). Loops were preloaded to 6 N to avoid potential errors caused by slack in the loops or by stretching of suture materials and to provide a well-defined starting point for data recording.

LTF testing was performed for both in vitro and in situ conditions using 10 samples of each suture–knot configuration for each mechanical testing. Each type of testing was conducted for a total of 240 suture–knot combinations per investigator. For the in vitro condition, each suture loop was initiated with 5 preconditioning loading cycles, from 6 N to 30 N at 1 Hz. The load was then applied continuously at a crosshead speed of 1 mm/s until “clinical failure” (3 mm crosshead displacement). We used this criterion for clinical failure, as studies have indicated that 3 mm is the point at which tissue apposition is lost.15,18-21 After the crosshead reached the 3-mm displacement, the loads (under load control) were held for 5 minutes at maximum load, and then load was applied continuously at a crosshead speed of 1 mm/s until complete structure failure. Load and displacement data were collected at a frequency of 20 Hz.

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